Quantification of polyphenolic compounds and flavonoids in Achillea millefolium and Equisetum arvense

Flavonoids have been of considerable importance and interest because of their medicinal activity. Responding to their numerous health benefits, a comparative study on the quantitative determination of total polyphenolic compounds and flavonoids was carried out in Achillea millefolium and Equisetum arvense. Total polyphenolic compounds were quantified by Folin-Ciocalteau method using different solvents in order to prove their extraction efficiency. Focus within total polyphenolic quantification study was placed on the traditional reflux and solvents used were: water, 100% acetone, 100% ethanol, 80% ethanol, 50% methanol and 70% methanol. In order to make flavonoids free from glycosidic moiety for quantification, hydrolysis was performed in 50% MeOH at 90°C using 6 M HCl concentration. Reverse phase high performance liquid chromatography [RP-HPLC] in gradient elution mode at 50°C using Hypersil BDS [RP-18] column was employed for the separation of flavonoids. Mobile phase used consisted of different combinations of water-methanol-tetrahydrofuran-phosphoric acid. Flavonoids quantified were luteolin, quercetin, apigenin, isorhamnetin and kaempferol

Determination of total polyphenolic compounds and flavonoids in Juglans regia leaves

Juglans regia leaves have been widely used in traditional medicines because of its antimicrobial, antihelmintic, astringent, keratolytic, antidiarrhoeal, hypoglycaemic, depurative, tonic, carminative activity. total polyphenolic compounds were determined using the Folin-Ciocalteau method and flavonoids were quantified using the HPLC-PDA after the hydrolysis of the plant material with HCI. Among the flavonoids myricetin, quercetin, apigenin and kaempferol were found in appreciable amount

Quality control of herbs: determination of amino acids in Althaea officinalis, Matricaria chamomilla and Taraxacum officinale

Analysis of raw materials and final products need reliable methods for the standardization of natural product drugs. Legal guideline also emphasizes on the qualitative and quantitative analyses of the plant constituents in an herbal product. In this study, thin layer chromatography [TLC] and amino acid analyzer was used for the determination of amino acids in plant extracts. Samples for this study were standards and aqueous extracts from Althaea officinalis, Matricaria chamomilla and Taraxacum officinale. Different amino acids in the extracts were detected through TLC. An automatic amino acid analyzer was used for the quantification of amino acids in the plant extracts under study.